BiotageIsolera One

Advanced and compact rapid preparation chromatography instrument

Biotage is the earliest company to develop a commercially available rapid preparative chromatograph. Biotage has been producing consumables such as chromatography columns since the 1980s and has been developing rapid preparation chromatography and its supporting products. In 1990, it launched a manual rapid preparation chromatography, a semi-automatic rapid preparation chromatography in 1992, and a fully automatic rapid preparation chromatography in 1996. Currently, over 11000 purification systems have been installed worldwide, with a leading market share. After acquiring Argonaut Technologies in 2005, Biotage's rapid preparative chromatography series has various types ranging from single channel to multi-channel, from laboratory research to industrial production, fully meeting the needs of different users.

Biotage Isolera rapid preparation chromatography series products are used to purify organic synthesis reaction systems, natural product extracts, and other organic mixtures.

Biotage Isolera One is a single channel system, while the 4-channel Isolera Four is an ideal multi-user system suitable for high-throughput laboratories. Isolera instrument, method editing is easy. By using its built-in touch screen or remote control from the office through a computer, you can edit gradients (click, drag points and lines), flow rates, collection volumes, detection wavelengths, and collection modes. You can also add more test tube supports during the purification process, which can be edited online for greater flexibility and intelligence.

Combined with the powerful Isolera Spektra software, Isolera One/Fun has advanced rapid preparation chromatography technology. Currently, only expensive or specially customized chromatography systems on the market possess these advanced features. For example, DAD scanning and λ - Al technology can detect and record the absorption data of compounds eluted from Flash chromatography columns at all UV wavelengths, measure and display the UV spectra of compounds. Advanced baseline calibration function can help reduce product loss at small wavelengths and lower excess solvent consumption. Determine purity through PDA spectroscopy without the need for post column purity analysis.

Four channel binary gradient/online addition of modifier

In the experiment, we can use four solvents for the same purification, making it easier to purify complex systems. Unlike traditional binary gradients, in a purification experiment, a gradient method composed of four solvents can achieve the separation and preparation of lipophilic and strongly polar compounds.

Isolera One rapid preparation chromatograph can add a third solvent to a binary gradient mobile phase at a constant ratio. In this way, modifiers can be effectively added to improve purification efficiency.

Add modifier

Method can be set: Add a third solvent to the binary gradient mobile phase at a constant ratio. Maintain the solubility of the compound to avoid system overpressure caused by the precipitation of crystals.

Biotage chromatography columns are suitable for all

The Isolera Spektra One and Isolera Spektra Four rapid preparative chromatographs are suitable for SNAP, SNAP Ultra, ZIP, and ZIP Sphere chromatography columns, Various specifications ranging from 5g to 750g (1500g can be used for Isolera LS).

main features

TLC to Step Gradient Conversion Gradient Method

Gradient optimization function, saving up to 60% of solvents

Online DAD scanning

2D and 3D spectral analysis

Regarding Biotage ® Optimization of SNAP Ultra Column Method

λ - Al full wavelength detection

Baseline correction function

Reverse phase chromatography technology

Current Speed: 1–200 mL/min

With one instrument, method development and pilot scale experiments can be completed

The volume of fraction collection reaches 9.6 L

Biotage ® IsoleraTM Spektra Four can sequentially purify multiple samples

The purification method can be directly applied to other Isolera systems

Biotage Isolera One is a single channel system, while the 4-channel Isolera Four is an ideal multi-user system suitable for high-throughput laboratories. Isolera instrument, method editing is easy. By using its built-in touch screen or remote control from the office through a computer, you can edit gradients (click, drag points and lines), flow rates, collection volumes, detection wavelengths, and collection modes. You can also add more test tube supports during the purification process, which can be edited online for greater flexibility and intelligence.